Single Cell Technologies Core

Single cell sequencing technologies facilitate analysis of genomic, transcriptomic and other multi-omics data to uncover differences in cell populations and shed light on evolutionary changes. CELS-SCT provides affiliated researchers the opportunity to use single cell infrastructure to support their investigations. We consult on experimental design and prepare single cell libraries for downstream sequencing. Consultation, operation and maintenance of equipment is managed by Amit Kumar, MS.

Understanding sample heterogeneity at the single cell level has become an important component of leukemia research, providing unparalleled insight on unique cell populations and unveiling novel mechanisms of treatment resistance and relapse. The CELS-SCT has supported several impactful research programs and with many planned projects in the pipeline, will continue to be an essential addition to our core facilities. 

10x Genomics Chromium X

• Whole transcriptome (5'/3')
• Differential expression
• Chromatin accessibility (ATAC-Seq)
• Full length paired TCRs / BCRs
• Cell surface protein

Mission Bio Tapestri

• Genomics (targeted assays)
• Single nucleotide variants
• Copy number alterations
• Structural variants
• Cell surface protein

Takara iCELL8 cx

• Full length transcriptome sequencing
• Isoforms, splicing, gene fusions
• Single nucleotide variants
• Chromatin accessibility (ATAC-Seq)