GEMM Shared Resource

The Genetically Engineered Mouse Model (GEMM) Shared Resource is available for investigators to obtain genetically modified mouse models. Our aim is to assist St. Jude investigators in their research programs to further the mission of St. Jude. We employ state of the art approaches to generate genetically modified mice, including CRISPR/Cas9 and related technologies. The facility also provides gene-targeting service and validated ES cell lines suitable for gene targeting experiments, selectable marker plasmids for construction of gene-targeting vectors, and training in ES cell culture. It also isolates new ES cell lines from any wild type or genetically modified mouse line and, in exceptional cases, cultures and submits for injection external ES cell lines.

GEMM is indispensable as it provides a means by which investigators can study a gene of interest in vivo. This approach is crucial for the identification of therapeutic targets in pediatric disease. GEMM has been utilized by 104 investigators since its inception and acknowledged on over 350 peer-reviewed publications. The GEMM collaborates with the Center for Advanced Genome Engineering (CAGE) in the design of CRISPR/Cas9 generated mouse models. The resource has generated more than 1,000 genetically engineered mouse models to date, making it a significant contributor to translational research efforts at St. Jude.