Cytogenetics

The Cytogenetic Shared Resource provides essential expertise and cutting-edge equipment to scientists across the institution. This resource enables researchers to design, build, and utilize cell-based genetic assays, allowing them to directly visualize newly discovered genetic events contributing to cancer biology within individual cells. By observing the genome in its natural cellular environment, researchers can gain unique insights that might otherwise be overlooked. Our experienced cytogenetic staff collaborates with scientists throughout St. Jude, advancing research goals through their specialized knowledge and techniques.

The Cytogenetic Shared Resource has contributed to numerous groundbreaking discoveries, including the initial cloning of the ALK gene, uncovering novel mechanisms of oncogene activation through analysis of 3D chromatin dynamics, and investigating the formation of transcription condensates in cancer. Our capabilities extend beyond traditional cytogenetics to include immunofluorescence protein staining combined with both RNA and DNA FISH, which can be applied to cells from both culture and tissue samples.

The resource is uniquely positioned to enhance understanding of transcription processes and identify potential intervention points to control individual gene expression. This work contributes significantly to advancing cancer research and potential treatment strategies at St. Jude. Through our collaborative approach and state-of-the-art techniques, we continue to play a crucial role in the institution's mission to combat childhood diseases.